Anti-human IL23A Recombinant Antibody (TAB-620LC)
CAT#: TAB-620LC
This recombinant monoclonal antibody is a humanized monoclonal antibody that specifically recognizes human IL-23A and has potent affinity against IL23A. It also can be potentially used in the research of Crohn's disease, psoriasis, psoriatic arthritis and asthma.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human IL23A Antibody (TAB-620LC) in WB
Western blot analysis of TAB-620LC was performed by loading human IL23A protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-620LC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human IL23A Antibody (TAB-620LC) in DB
Dot Blot analysis of TAB-620LC was performed by coating with human IL23A protein.
TAB-620LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-Human IL23A Antibody (TAB-620LC) in ELISA
ELISA analysis of TAB-620LC was performed by coating with human IL23A protein. Then blocked with BSA and incubated with TAB-620LC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 4 Anti-human IL23A Recombinant antibody (TAB-620LC) in SDS-PAGE
SDS-PAGE analysis of TAB-620LC in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 5 Anti-human IL23A Recombinant antibody (TAB-620LC) in HPLC
HPLC analysis of TAB-620LC shows the purity is>95%.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells in and outside germinal center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_8_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/60_H7_1_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110944-IL23A
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Applications
- ELISA
- Related Disease
- Crohn's disease, psoriasis, psoriatic arthritis and asthma
Target
- Alternative Names
- IL23A; interleukin 23, alpha subunit p19; P19; SGRF; IL-23; IL-23A; IL23P19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
- Gene ID
- 51561
- UniProt ID
- Q9NPF7
REVIEWS AND Q&AS
CITATIONS
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Customer Review
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Reliable Detection
I've been using the Anti-human IL23A Recombinant Antibody for my ELISA assays, and it consistently provides reliable results. Highly recommended for researchers needing precise IL23A detection.
Consistent Quality
The batch-to-batch consistency of this antibody is remarkable. Each purchase provides the same high quality and specific binding in my assays. Very dependable.
Easy to Use
The Anti-human IL23A Recombinant Antibody is user-friendly with detailed protocols provided. It fit seamlessly into my existing workflows, making my research more efficient.
Q&As
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What is the recommended concentration for ELISA applications using this antibody?
A: It is generally recommended to use a concentration range of 0.1-2 µg/mL; however, depending on the particular experimental conditions, optimization can be required.
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What species reactivity does this antibody exhibit?
A: The specific target of this recombinant antibody is human IL23A.
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Can this antibody neutralize IL23A activity?
A: Its purpose as a research tool is to bind and detect IL23A. Although neutralizing activity might be seen, more functional validation in your particular application is advised.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-620LC, RRID: AB_3111979)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| PFBL-241 | Human Anti-IL23A Recombinant Antibody (clone CNTO1959); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-0860-FY-S(P) | Mouse Anti-IL23A Recombinant Antibody; scFv Fragment (HPAB-0860-FY-S(P)) | Inhib | Mouse scFv |
| HPAB-0861-FY-S(P) | Mouse Anti-IL23A Recombinant Antibody; scFv Fragment (HPAB-0861-FY-S(P)) | Inhib | Mouse scFv |
| HPAB-0865-FY-S(P) | Mouse Anti-IL23A Recombinant Antibody; scFv Fragment (HPAB-0865-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0866-FY-S(P) | Mouse Anti-IL23A Recombinant Antibody; scFv Fragment (HPAB-0866-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-605LC | Anti-human IL23A Recombinant Antibody (FM303) (TAB-605LC) | ELISA | Humanized antibody |
| TAB-608LC | Anti-human IL23A Recombinant Antibody (21-4) (TAB-608LC) | ELISA | Humanized antibody |
| TAB-609LC | Anti-human IL23A Recombinant Antibody (8-22) (TAB-609LC) | ELISA | Humanized antibody |
| TAB-610LC | Anti-human IL23A Recombinant Antibody (6-22) (TAB-610LC) | ELISA | Humanized antibody |
| TAB-611LC | Anti-human IL23A Recombinant Antibody (1-4) (TAB-611LC) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-606LC | Anti-human IL23A Recombinant Antibody (E11E7) (TAB-606LC) | ELISA, Neut | |
| TAB-614LC | Anti-human IL23A Recombinant Antibody (8C9) (TAB-614LC) | ELISA, Inhib | |
| TAB-615LC | Anti-human IL23A Recombinant Antibody (2H6) (TAB-615LC) | ELISA, Inhib | |
| TAB-621LC | Mouse Anti-IL23A Recombinant Antibody (TAB-621LC) | ELISA, WB, Neut | Mouse IgG |
| TAB-606LC-S(P) | Anti-human IL23A scFv Fragment (E11E7) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-607LC | Anti-human IL23A Recombinant Antibody (E11E7Chimera) (TAB-607LC) | ELISA, Neut, FuncS | Chimeric antibody (rat/human) |
| TAB-607LC-S(P) | Anti-human IL23A scFv Fragment (E11E7Chimera) | ELISA | Chimeric antibody (rat/human) |
| TAB-607LC-F(E) | Anti-human IL23A Fab Fragment (E11E7Chimera) | ELISA | Chimeric antibody (rat/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-613LC | Anti-human IL23A Recombinant Antibody (ST883/885) (TAB-613LC) | ELISA | Human antibody |
| TAB-622LC | Human Anti-IL23A Recombinant Antibody (TAB-622LC) | ELISA | Human antibody |
| TAB-613LC-S(P) | Anti-human IL23A scFv Fragment (ST883/885) | ELISA | Human antibody |
| TAB-622LC-S(P) | Human Anti-IL23A Recombinant Antibody; scFv Fragment (TAB-622LC-S(P)) | ELISA | Human scFv |
| TAB-008ML | Anti-Human IL23A Recombinant Antibody (Brazikumab) | ELISA, IHC, FC, IP, IF, Inhib | IgG2, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1335CQ | Mouse Anti-IL23A Recombinant Antibody (NEUT-1335CQ) | FuncS, Neut | Mouse IgG1, κ |
| NEUT-1336CQ | Rabbit Anti-IL23A Recombinant Antibody (VMC198) | WB, Neut | Rabbit IgG2b |
| NEUT-1337CQ | Human Anti-IL23A Recombinant Antibody (clone CBL1018) | Neut | Human IgG1, κ |
| NEUT-1338CQ | Mouse Anti-IL23A Recombinant Antibody (NEUT-1338CQ) | Neut | Mouse IgG1 |
| NEUT-1339CQ | Mouse Anti-Il23a Recombinant Antibody (clone CBL1028) | Neut | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4335 | Rabbit Anti-IL23A Recombinant Antibody (clone SI424DS) | ELISA | Rabbit IgG |
| MOR-4615 | Rabbit Anti-IL23A Recombinant Antibody (clone TH128DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-875 | Afuco™ Anti-IL23A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-875) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-620LC | Afuco™ Anti-IL23A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-620LC) | ELISA | ADCC enhanced antibody |
| AFC-TAB-008ML | Afuco™ Anti-IL23A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-008ML) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0860-FY | Mouse Anti-IL23A Recombinant Antibody (HPAB-0860-FY) | WB | Mouse IgG |
| HPAB-0861-FY | Mouse Anti-IL23A Recombinant Antibody (HPAB-0861-FY) | Inhib | Mouse IgG |
| HPAB-0865-FY | Mouse Anti-IL23A Recombinant Antibody (HPAB-0865-FY) | ELISA | Mouse IgG |
| HPAB-0866-FY | Mouse Anti-IL23A Recombinant Antibody (HPAB-0866-FY) | ELISA | Mouse IgG |
| HPAB-0867-FY | Mouse Anti-IL23A Recombinant Antibody (HPAB-0867-FY) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1120 | Anti-IL23A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3514 | Anti-Human IL23A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY128 | Human Anti-IL23A (clone AMG 139) scFv-Fc Chimera | Inhib, ELISA, FC | Human IgG1, scFv-Fc |
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